SMS logo
Format Conversion
-Combine FASTA
-EMBL to FASTA
-EMBL Feature Extractor
-EMBL Trans Extractor
-Filter DNA
-Filter Protein
-GenBank to FASTA
-GenBank Feature Extractor
-GenBank Trans Extractor
-One to Three
-Range Extractor DNA
-Range Extractor Protein
-Reverse Complement
-Split FASTA
-Three to One
Sequence Analysis
-Codon Plot
-Codon Usage
-CpG Islands
-DNA Pattern Find
-DNA Stats
-Fuzzy Search DNA
-Fuzzy Search Protein
-Ident and Sim
-Multi Rev Trans
-Mutate for Digest
-ORF Finder
-Pairwise Align Codons
-Pairwise Align DNA
-Pairwise Align Protein
-PCR Primer Stats
-PCR Products
-Protein GRAVY
-Protein Isoelectric Point
-Protein Molecular Weight
-Protein Pattern Find
-Protein Stats
-Restriction Digest
-Restriction Summary
-Reverse Translate
-Translate
Sequence Figures
-Color Align Conservation
-Color Align Properties
-Group DNA
-Group Protein
-Primer Map
-Restriction Map
-Translation Map
Random Sequences
-Mutate DNA
-Mutate Protein
-Random Coding DNA
-Random DNA Sequence
-Random DNA Regions
-Random Protein Sequence
-Random Protein Regions
-Sample DNA
-Sample Protein
-Shuffle DNA
-Shuffle Protein
Miscellaneous
-IUPAC codes
-Genetic codes
-Browser compatibility
-Mirror this site
-Use this site off-line
-About this site
-Acknowledgments
-Reference

The Sequence Manipulation Suite Copyright © 2000, 2004 Paul Stothard. Send questions and comments to stothard@ualberta.ca
home
Sequence Manipulation Suite:
Mutate for Digest
Mutate for Digest accepts a DNA sequence as input and searches for regions that can easily be mutated to create a restriction site of interest. The program also displays protein translations so that you can see which reading frames are altered by the proposed mutations. Use Mutate for Digest to find sequences that can be converted to a useful restriction site using PCR or site-directed mutagenesis.

Paste a raw sequence or one or more FASTA sequences into the text area below. Input limit is 10000 characters.

Please check the browser compatibility page before using this program.
  • Search for future sites.
  • Show bases per line.
  • Show the translation for reading frame
  • Use the genetic code.
*This page requires JavaScript. See browser compatibility.
*You can mirror this page or use it off-line.

new window | home | citation
2.4.4-Sat May 12 05:03:03 2007
Valid XHTML 1.0! Valid CSS!